Composite

Part:BBa_K1366101:Design

Designed by: Eduardo Cepeda Cañedo, Mercedes Alejandra Vazquez Cantu   Group: iGEM14_Tec-Monterrey   (2014-10-10)


Genetic construct for lpp gene deletion (C1)


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal XbaI site found at 44
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 1202
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal XbaI site found at 44
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal XbaI site found at 44
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

This is the general structure 50bp homology Lpp // FRT // Amp R Promoter // RBS // AmpR // Terminator // FRT // MCS // 50 bp homology Lpp


Source

The genetic design is based in the lpp deletion done by: Ni, Y., Reyes, J., & Chen, R. (2007). lpp Deletion as a Permeabilization Method. Biotechnology and Bioengineering, 1347-1356. It also contains some parts of the registry like BBa_K1073000, BBa_B0034, BBa_B0015

References